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The project requires the utilization of the most advanced and costly molecular techniques and comprises six phases
EE1: The pre-analytical phase includes the collection of information about the history of the sample, the systematic evaluation of the phenotype and the realization of the necessary samplings. For the evaluation and selection of the sample, information will be collected on livestock holdings and farmed animals using a special questionnaire. In addition, a systematic evaluation of the phenotype will be carried out based on zootechnical criteria for the classification of the goats in each breed. Finally, milk samples will be taken from the goats of the main breeds of Skopelos, domestic, improved mixes, Serres, Pangaios and Aridaias.
Deliverable: 1. Data – report on tribes
E.E.2: The analytical phase involves a number of laboratory steps including “library” construction, target enrichment and sequencing reaction. It also includes three levels of data analysis, primary, secondary and tertiary analysis and categorization of the extracted variants.
Deliverable:
1. Genome recombination
2. Genotyping microarrays
E.E.3: The whole genome sequencing (Whole Genome Sequencing-WGS) from representative samples of the 6 goat breeds of North Macedonia and the use of genotyping microarrays for comparison with samples from foreign breeds, they will lead to the identification of traceability indicators of milk and its products and the emergence of a national identity of dairy products.
Deliverables:
1. Traceability indicators
2. Report of findings of the meta-analytic phase.
E.E.4: The accumulation of the above results will lead to the development of new molecular methods, targeted and affordable, which could be applied to a large number of samples in order to determine the repeatability and reliability of the indicators that will result from the analyses.
Deliverable: 1. Milk goat kit
E.E.5: There will be a detection of exogenous sugars (fructose, sucrose, etc.) that can be added to milk together with water and milk proteins in order to increase the quantity of milk and increase the financial profit of those who sell the milk. The detection of these sugars, both qualitatively and quantitatively, will be based on High Performance Liquid Chromatography and/or Mass Chromatography with an LC MS-MS chromatograph.
Deliverable: 1. Tamper detection protocol
E.E.6: Next, genetic traceability of milk and its products will be carried out. The tools developed will be applied to processed products such as butter, yogurt – yogurt desserts, white cheeses, hard cheeses, curd cheeses. The experimental application of these tools will enable us to certify with certainty the origin of the milk used as raw material and to ensure that it comes exclusively from domestic goat breeds.
Deliverables: 1. Traceability system
Project director:
sakaridis@elgo.gr
